rabbit polyclonal anti human fpr3 antibody (Biorbyt)
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Biorbyt
rabbit polyclonal anti human fpr3 antibody
Rabbit Polyclonal Anti Human Fpr3 Antibody, supplied by Biorbyt, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+human+fpr3+antibody/FPR3+antibody/pmc08555499-112-0-6
Average 92 stars, based on 4 article reviews
Rabbit Polyclonal Anti Human Fpr3 Antibody, supplied by Biorbyt, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+human+fpr3+antibody/FPR3+antibody/pmc08555499-112-0-6
Average 92 stars, based on 4 article reviews
rabbit polyclonal anti human fpr3 antibody - by Bioz Stars,
2026-10
92/100 stars
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1) Product Images from "Identification and validation of candidate genes dysregulated in alveolar macrophages of acute respiratory distress syndrome"
Article Title: Identification and validation of candidate genes dysregulated in alveolar macrophages of acute respiratory distress syndrome
Journal: PeerJ
doi: 10.7717/peerj.12312
Figure Legend Snippet: (A) PPI network was constructed basing on the top 300 DEGs. The red icons represent up-regulated genes and the blue ones represent down-regulated genes. Furthermore, the connecting lines between the icons indicate potential interaction between different DEGs. (B) Module analysis of the DEGs enrolled in PPI network using MCODE method. The top five ranking clusters are shown according to MCODE score. (C) Functional enrichment analysis of genes in cluster 1. Likewise, the y axis and x axis represent enrichment terms and scores, respectively, and the color means p- value. (D) The top 10 hub genes are identified from PPI network using MCC method. The color represents MCC score. A darker red icon means higher MCC score and a lighter yellow one represents lower MCC score. (E) mRNA expression levels of CCL13, CCR2, FPR3 and PLAU in alveolar macrophages isolated from better prognosis subjects (VFDs > 12) and from poor prognosis individuals (VFDs <12).
Techniques Used: Construct, Functional Assay, Expressing, Isolation
Figure Legend Snippet: (A) Relative mRNA expressions of CCL13, CCR2, CX3CR1, CXCL16, FPR3 and PLAU on THP-1-derived macrophages in response to LPS were assessed using qRT-PCR. (B) The protein levels of CCR2 and FPR3 under a varying time of LPS treatment were evaluated by Western blot analysis. (C) CCR2 expression on surface of THP-1-derived macrophages after LPS treatment was detected by flow cytometry analysis. (D) Relative mRNA expressions of CCR2 and FPR3 on primary AMs collected from patients and volunteers were evaluated using qRT-PCR. (E) The protein levels of CCR2 and FPR3 in primary AMs were examined using Western blot assay. (F) CCR2 expression on surface of primary AMs was detected by flow cytometry analysis. Data are presented as mean ± SD and are representative of three independent experiments. ∗ P < 0.05, by unpaired, 2-tailed Student’s t test.
Techniques Used: Derivative Assay, Quantitative RT-PCR, Western Blot, Expressing, Flow Cytometry
Figure Legend Snippet: (A)Diagrammatic illustration for the chemotaxis assay procedure: Upper:1 ×10 4 of wild-type or siRNA-transfected macrophages were cultured in serum-free medium with or without LPS. Lower: Culture medium with or without CCL2/fMLP. (B) LPS-induced macrophage chemotaxis toward chemokine CCL2 was assessed using a transwell assay as described in the Methods. (C) Likewise, LPS-triggered macrophage migration toward fMLP was evaluated as above. (D) The correlation between CCR2 and FPR3 expression was analyzed using pearson correlation analysis with expression data of GSE89953 and GSE116560 . (E) Elevated protein expression of CCR2 induced by LPS stimulation was significantly attenuated by siRNA-FPR3 transfection. Data are presented as mean ± SD and are representative of 3 independent experiments. ∗ P < 0.05, by unpaired, two-tailed Student’s t test.
Techniques Used: Chemotaxis Assay, Transfection, Cell Culture, Transwell Assay, Migration, Expressing, Two Tailed Test
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